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Evaluation of neutrophil alkaline phosphatase (NAP) activity: Untreated myeloid leukaemia, lymphoid leukaemia and normal humans
Author(s) -
BendixHansen Knud,
HellebergRasmussen Inge
Publication year - 1985
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1985.tb02790.x
Subject(s) - nap , chronic myeloid leukaemia , medicine , myeloid leukaemia , alkaline phosphatase , myeloid , confidence interval , leukemia , immunology , gastroenterology , biology , enzyme , biochemistry , neuroscience
Neutrophil alkaline phosphatase (NAP) activity was estimated in 50 healthy humans and 89 patients with leukaemia; 41 cases of acute myeloid leukaemia (AML), 22 cases of chronic myeloid leukaemia (CML), and 26 cases of lymphoid leukaemia (LL). The groups proved to be separate entities (p < 0.000 25) and the differences between the groups were statistically significant (p < 0.001) except for the difference between AML and LL. The 95% confidence limits for normal NAP scores were 15.0–132.6. Decreased scores were demonstrated in 73% of CML, 7% of AML but never in LL patients. Increased scores were found in 37% of AML, 31% of LL but never in CML patients. Evaluation of the distribution of the single cell NAP acivity (negative, weak positive, strong positive) showed decreased activity in 77% of CML, 15% of AML but never in LL patients. Increased activity was demonstrated in 63% of AML, 54% of LL and 9% of CML patients. The evaluation of single cell activity is a time‐saving method, which furthermore proved superior to the scoring method in discriminating between the types of leukaemia investigated.