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Myelopoiesis and erythropoiesis of bone marrow cells cultured in vitro in patients recovered from aplastic anaemia
Author(s) -
Ruvidić R.,
Jovčić G.,
BiljanovićPaunović L.,
Stojanović N.,
Mijović A.,
PavlovićKentera V.
Publication year - 1985
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1985.tb02268.x
Subject(s) - myelopoiesis , erythropoiesis , erythropoietin , bone marrow , cfu gm , progenitor cell , in vitro , stem cell , aplastic anemia , haematopoiesis , immunology , andrology , biology , medicine , microbiology and biotechnology , endocrinology , anemia , biochemistry
Methylcellulose culture assay was used to detect committed haemopoietic stem cells, CFU‐C and CFU‐E, in aplastic anaemia patients with autologous haemopoietic reconstitution. Severe diminution of CFU‐C was found in all the patients studied and the absence of a dose‐response to colony stimulating factor (CSF) was demonstrated. A reduced number of CFU‐E and lower erythropoietin (Ep) sensitivity of those progenitors was detected as well. Autologous serum added to the bone marrow cultures of these patients enhanced the growth of CFU‐C but inhibited CFU‐E growth. According to the results presented, some residual damage at the stem cell level is suggested.