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Cyclosporin A (CyA) does not enhance CFU‐c growth in patients with severe aplastic anaemia
Author(s) -
Bacigalupo A.,
Frassoni F.,
Podesta M.,
Piaggio G.,
Lint M.T.,
Repetto M.,
Marmont A.M.
Publication year - 1985
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1985.tb02245.x
Subject(s) - bone marrow , in vitro , medicine , immunology , rosette (schizont appearance) , andrology , microbiology and biotechnology , chemistry , gastroenterology , biology , biochemistry
8 patients with severe aplastic anaemia (SAA) in remission following immunosuppres‐sive therapy were studied for CFU‐c growth from unfractionated or E‐rosette depleted (E‐) bone marrow (BM) cells. Cyclosporin A (CyA) was added to unfractionated BM cells at a concentration of 1000 ng/ml. The mean number of CFU‐c/10 5 BM cells plated was 6 ± 6 from undractionated BM cells, 28 ± 20 from E‐BM cells, and 8 ± 7 from unfractionated BM cells supplemented with CyA. All patients had significant increase of CFU‐c growth after E rosette depletion (overall P = 0.002). On the contrary, only 1 patient showed an increase of CFU‐c growth after addition of CyA, and overall there was no difference between untreated and CyA treated BM cells (P = 0.7). These results suggest that addition of CyA to BM cells in vitro is not an effective means of enhancing CFU‐c growth in SAA patients.