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Effect of sera from various vertebrate species on mouse erythroid progenitor proliferation
Author(s) -
Butin Christophe,
Blanchet Jean Paul
Publication year - 1985
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1985.tb01722.x
Subject(s) - vertebrate , spleen , erythropoiesis , biology , antibody , progenitor cell , microbiology and biotechnology , chemistry , immunology , biochemistry , medicine , stem cell , anemia , gene
Sera from different vertebrate species were tested for their stimulating activity upon mouse CFU‐E growth. Crude sera were either naturally stimulating or toxic. Absorption on mouse blood and spleen cells or decomplementation removes or neutralizes the toxicity, probably mediated through natural anti‐mouse antibodies. All but 2 sera tested (ox and sheep) were stimulatory. This stimulating activity was found to be either heat‐sensitive or ‐resistant according to the sera. Upon chromatography on DEAE cellulose, 2 fractions were obtained which were both stimulatory. Whether these two stimulating activities correspond to different molecules or to only 1 absorbed on different serum proteins remains to be determined. These results confirm previous results obtained for mouse and human sera and suggest that this cross‐reactive stimulating activity may have an important role in vertebrate erythropoiesis.