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Functional and multimarker analysis of T‐cell chronic lymphocytic leukaemia
Author(s) -
Baldini L.,
Padova F.,
Cortelezzi A.,
Neri A.,
Nobili L.,
Lavezzi A. M.,
Maiolo A. T.,
Polli E. E.
Publication year - 1985
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1985.tb00749.x
Subject(s) - immunophenotyping , lymphocytosis , immunology , chronic lymphocytic leukemia , biology , medicine , flow cytometry , leukemia
The morphology, immunophenotype, cytoenzymatic and functional activities of T lymphocytes from 4 patients with chronic lymphoproliferative disease of T‐cell origin were studied. Clonal proliferation was demonstrated by distinctive chromosomal abnormalities involving chromosomes 2 and 14. Patients 1 and 2 were classifiable as OKT4+ T‐cell chronic lymphocytic leukaemia (T‐CLL) and patient 3 as OKT4+/OKT8+ T‐CLL, with helper function in vitro only in patient 1. Patient 4 has low‐grade lymphocytosis with benign clinical course, with cells showing morphology of large granular lymphocytes (LGL), and immunophenotype HNK‐1+, ER+, Fcy receptor+, OKT3+, OKT11+ and OKT8+, as well as natural killer activity, radiosensitive suppressor activity on Ig secretion and responsiveness to PHA; this case was interpreted as LGL leukaemia. This study indicates that a large proportion of cases of true T‐CLL may belong to the OKT4 subset, and that extensive investigations should be made of the lymphocytic OKT8+/Tγ forms to characterize them precisely.