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Application of the Beckman JE6‐B Elutriator System® in the isolation of human monocyte subpopulations
Author(s) -
Mason Richard R.,
Weiner Roy S.
Publication year - 1985
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1985.tb00735.x
Subject(s) - monocyte , rotor (electric) , flow (mathematics) , significant difference , chemistry , zoology , immunology , medicine , physics , biology , mechanics , quantum mechanics
Beckman Instruments recently developed an improved version of their JE‐6 Elutriator Rotorr̀. At 3000 rpm (867 x g), lymphocytes were eluted from the chamber at flow rates of 10 to 18 ml/min. Small monocytes (362 μ 3 ) were collected at 18 ml/min over 400 ml while the larger monocytes (395 μ 3 ) were retained in the chamber until the flow rate was increased to 28 ml/min. These parameters differ from those previously reported by us when we used Beckman's original commercial version of the JE‐6 Elutriator Rotor. The new JE‐6B rotor required an increase of centrifugal force from 625 to 867 x g generated by an increase in rpm from 2500 to 3000, as well as an increase in flow rates to collect both small (17 to 18 ml/min) and large (26 to 28 ml/min) monocytes. The monocytes collected using the new rotor with the revised protocol have the same physical and functional properties as those collected with the earlier version using our previously published protocol.