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METHODOLOGY OF THE TWO‐STAGE ASSAY OF FACTOR VIII (VIII:C)
Author(s) -
Barrowcliffe Trevor W.
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb02765.x
Subject(s) - chromogenic , stage (stratigraphy) , reagent , automation , chromatography , computer science , chemistry , engineering , biology , mechanical engineering , paleontology
The principle of the two‐stage assay, the preparation of reagents and the performance of the assay are described in detail. Modifications introduced by various authors are discussed, including a simplified version designed for automation, and a chromogenic assay. Finally, examples are given of the methodology used in our laboratory.