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A Tissue Culture Model of Cartilage Breakdown In Haemophilic Arthropathy
Author(s) -
Smith P.D. McLardy,
Ashton I.K.,
Duthie R.B.
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb02567.x
Subject(s) - cartilage , rheumatoid arthritis , medicine , tissue culture , glycosaminoglycan , arthropathy , osteoarthritis , immunology , endocrinology , pathology , chemistry , anatomy , biochemistry , in vitro , alternative medicine
Synovium in tissue culture has a degradative effect on cartilage which is partly chemically mediated. This effect has been shown to be more marked in the inflammatory arthropathies. The possibility of similar effects of haemophilic synovium were investigated. Synovial tissue from patients with either chronic haemophilic arthropathy, rheumatoid arthritis or no inflammatory arthropathy was cultured. The prostaglandin production of the tissue in culture, and the effects of the haemophilic synovium conditioned medium (HCM) on human cartilage were compared. Results show that haemophilic synovium produces prostaglandin E in culture, at markedly higher levels (upto 872 pg/mg synovium/hour n = 11) compared with normal (maximum 217 pg/mg/hour; n = 3). The HCM also stimulated glycosaminoglycans breakdown and decreased retention of newly synthesised chondroitin sulphate (CS) in cartilage cultured in it when compared to the normal conditioned medium (NCM). Expressed as a percentage of CS in the whole culture system after 14 days co‐culture, CS lost from the cartilage compared to controls was 11.7% (S.E. 3. 4, n = 6) for HCM and 1.8% (S.E. 0.6 n = 4) for NCM. In comparison in two rheumatoid conditioned media (RCM) the loss was 14.7% and 12.6%. a similar effect has been demonstrated for haemophilic synovium as has previously been reported for rheumatoid synovium.