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Freezing of Plasma to Obtain Better Yield of Factor VIII:C
Author(s) -
Carlebjörk G,
Blombäck M
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb02554.x
Subject(s) - ampoule , chemistry , freezing point , thermocouple , chromatography , fresh frozen plasma , yield (engineering) , analytical chemistry (journal) , materials science , composite material , thermodynamics , medicine , physics , platelet
Plasma was frozen in two types of ampoules with different freezing equipments. Changes in temperature were recorded with thermocouples and the quality of frozen plasma determined by analysis of F VIII:C. Temperature decreased rapidly to the freezing point where it stayed during the phase change to ice and was followed by a second rapid decrease down to the final temperature. The time for duration at the freezing point decreased with thinner plasma layers. Freezing was accomplished faster with ‐40°C ethanol baths than with different types of freezeboxes. Use of flat 750 ml ampoules resulted in shorter freezing times than with cylindric 1,500 ml ampoules. The factor VIII:C recovery in frozen plasma increased with faster rate of freezing. The purity and recovery of F VIII:C in cryoprecipitates increased with fast freezing of plasma.