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Lactoferrin receptors in normal and leukaemic human blood cells
Author(s) -
Birgens Henrik S.,
Karle Hans,
Hansen Niels Ebbe,
Kristensen Lars Østergaard
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb02228.x
Subject(s) - lactoferrin , lymphoblast , receptor , peripheral blood , microbiology and biotechnology , mole , chemistry , platelet , immunology , biology , biochemistry , cell culture , genetics
The binding of 125 I‐lactoferrin to a variety of peripheral blood cells was examined. In the concentration range from 10 −9 mol/l to 10 −6 mol/l a receptor‐like binding of lactoferrin was observed in monocytes as well as in polymorphonuclear leucocytes. At the low concentrations of lactoferrin in plasma (about 10 −9 mol/l) the cellular binding to monocytes was about 10 times higher than the binding to polymorphonuclear leucocytes and lymphocytes. A major result from a kinetic analysis was a lower apparent binding affinity to polymorphonuclear leucocytes (K D about 2 times 10 −7 mol/l) than to lymphocytes and monocytes (K D about 2 times 10 −8 mol/l). Studies in leukaemic cells showed that lymphocytes from patients with chronic lymphocytic leukaemia bound lactoferrin to the same small extent as normal lymphocytes. In contrast, a larger component of binding with high affinity (K D about 2 times 10 −8 mol/l) could be demonstrated to lymphoblasts as well as to myeloblasts.