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Immunohistochemical identification of lymphocyte subsets and accessory cells in human hyperplastic lymph nodes The functional significance of the compartmentalization of lymphoid tissue
Author(s) -
Ralfkiær Elisabeth,
Plesner Torben,
Wantzin Gunhild Lange,
Thomsen Kristian,
Nissen Nis I.,
HouJensen Klaus
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb02197.x
Subject(s) - calla , pathology , germinal center , mantle zone , follicular dendritic cells , monoclonal antibody , biology , lymph , bone marrow , lymph node , histiocyte , antibody , microbiology and biotechnology , antigen presenting cell , immunology , t cell , b cell , medicine , immune system
Biopsies from 21 lymph nodes with benign hyperplasia were examined by immuno‐enzymatic labelling of frozen sections with a panel of monoclonal antibodies. B‐cells (B1 + , HLA‐DR + , C3b receptor +/‐ ) localized in primary follicles, secondary follicles, and areas adjacent to the subcapsular sinus. The B‐cells in primary follicles and mantle zones of secondary follicles were indistinguishable (SmIgD + , SmIgM + , Cylg − , T10 − , CALLA − ). B‐cells adjacent to the subcapsular sinus demonstrated a higher density of SmIgM, and a weaker expression of SmIgD. The germinal centre cells showed a more differentiated phenotype (SmIgD − , SmIgM + , CyIgM +/‐ ), and also expressed T10 and CALLA. T‐cells (Lyt3 + Lyt2 + , Leu4 + , OKT6 − , OKT10 − ) localized in paracortial and interfollicular areas, and demonstrated a relative predominance of T‐helper/inducer cells (Leu3 + ). T‐helper/inducer cells were also identified in secondary follicles. The B‐cell areas contained dendritic reticulum cells (R4/23 + , C3b‐receptor + ). Interdigitating reticulum cells (HLA‐DR + , OKT6 +/‐ ) localized in T‐cell regions. The cells in sinuses demonstrated monocyte/macrophage properties (MO2 + , Ig + , C3b‐receptor + , HLA‐DR +/‐ ).