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Demonstration of Histamine Receptors on Human Platelets by Flow Cytometry
Author(s) -
Hallberg Torgny,
Dohlsten Mikael,
Baldetorp Bo
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb02165.x
Subject(s) - histamine , platelet , receptor , histamine receptor , cimetidine , histamine h1 receptor , histamine h4 receptor , chemistry , pharmacology , histamine h2 receptor , diphenhydramine , flow cytometry , population , receptor antagonist , antagonist , endocrinology , medicine , immunology , biology , biochemistry , environmental health
Fluoresceinated human albumin conjugated with histamine (FHA‐HIS) has been used for the demonstration of histamine receptors on human platelets. Such receptors were demonstrated on 40–63% of peripheral blood platelets in 4 healthy donors. The binding of FHA‐HIS was inhibited on 35–79% of the platelets by the histamine H 1 receptor antagonists diphenhydramine and clemastine. The histamine H 2 receptor antagonist cimetidine blocked the FHA‐HIS binding on 14–37% of the platelets. It is concluded that histamine H 1 as well as H 2 receptors occur on human platelets but the receptors are not equally distributed in the platelet population.