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Expression of spectrin in normal and malignant erythropoiesis
Author(s) -
Ekblom Marja
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb00711.x
Subject(s) - spectrin , glycophorin , erythropoiesis , haematopoiesis , biology , microbiology and biotechnology , phenotype , immunology , antigen , cell , genetics , medicine , gene , cytoskeleton , anemia , stem cell
Spectrin is a major constituent of the erythrocyte membranoskeleton. The occurrence of spectrin during normal and malignant erythropoiesis was investigated by immuno‐fluorescence using a monospecific rabbit anti‐human spectrin antiserum. The expression of spectrin was correlated to the presence of glycophorin A, which is an early and specific marker for erythroid cells. The expression of spectrin during normal erythroid differentiation coincided with that of glycophorin A. Both markers were already present in the proerythroblasts. Spectrin was also found in leukaemic cells from patients with acute erythroleukaemia and erythroid blast crisis of chronic myelogenous leukaemia. In a large panel of human haematopoietic cell lines only those with erythroid phenotype (K 562 and HEL) stained positively for spectrin. It is concluded that spectrin appears early in the erythroid maturation. It is expressed both in normal and malignant erythroid precursors. Spectrin can be used as a marker for erythroid blasts in the diagnosis of erythroleukaemias.—