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Yeast cell phagocytosis by mononuclear leucocytes from peripheral blood: Significance of the substrate
Author(s) -
Rydgren Lars,
Norberg Bo
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb00709.x
Subject(s) - phagocytosis , yeast , peripheral blood mononuclear cell , substrate (aquarium) , peripheral blood , chemotaxis , cell , microbiology and biotechnology , chemistry , biology , immunology , in vitro , biochemistry , ecology , receptor
Lymphoprep®‐isolated mononuclear leucocytes (L‐MNs) from peripheral blood of healthy donors were mixed with yeast cells and filmed by time‐lapse microcinemato‐graphy in glass chambers at +37°C. The concentration of L‐MNs and yeast cells was lowered so as to make directional L‐MN movement (chemotaxis) a necessary conditon of phagocytosis. 54 cases of phagocytosis were filmed. Only yeast cells lying immobile on the glass surface were engulfed. In addition, 11 collisions between free‐floating yeast cells and glass‐adherent L‐MNs were noted. None of these collisions led to immediate engulfment. It is suggested that the process of phagocytosis requires prolonged leucocyte‐prey contact, in the order of magnitude of 3 to 5 seconds.—