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Erythroid and granulocyte‐macrophage colony formation in myelodysplastic syndromes
Author(s) -
Ruutu Tapani,
Partanen Seija,
Lintula Riitta,
Teerenhovi Lasse,
Knuutila Sakari
Publication year - 1984
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1984.tb00695.x
Subject(s) - granulocyte , haematopoiesis , progenitor cell , bone marrow , karyotype , biology , macrophage , colony forming unit , immunology , myelodysplastic syndromes , agar , pathology , medicine , stem cell , genetics , in vitro , chromosome , gene , bacteria
Colony formation by haematopoietic progenitors from the bone marrow was studied in 44 patients with a myelodysplastic syndrome. Erythroid progenitors BFU‐E and CFU‐E were cultured in methyl cellulose, and granulocyte‐macrophage precursors CFU‐GM in agar. 3 of 32 patients showed normal numbers of BFU‐E colonies; in all the other cases the number of these colonies was below the normal range. CFU‐E colony formation was subnormal in all cases. 23 of 44 patients grew normal numbers of colonies and clusters in CFU‐GM cultures. These patients had refractory anaemia with ring sideroblasts (FAB‐classification) or 5q‐ karyotype anomaly in the marrow. Patients lacking both of these findings exhibited reduced colony formation or excessive growth of colonies and/or clusters, with few exceptions. In conclusion, we found that erythroid colony formation was defective in all cases. Normal granulocyte‐macrophage colony formation was associated with refractory anaemia with ring sideroblasts or the presence of 5q‐ karyotype anomaly.