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Myeloperoxidase‐Deficient Polymorphonuclear Leucocytes
Author(s) -
BendixHansen Knud,
Nielsen Henning Kaspersen
Publication year - 1983
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1983.tb02527.x
Subject(s) - myeloperoxidase , granulocyte , immunology , myeloid leukaemia , medicine , myeloid , pathology , inflammation
Neutrophil myeloperoxidase (MPO) activity was analysed semi‐quantitatively both by (i) MPO‐scoring of polymorphonuclear leucocytes (PMN) and (ii) counting the MPO‐deficient PMN (PMN lacking MPO) in 164 subjects (60 cases of leukaemia and 104 normal humans). The scoring method showed that 10 out of 21 (48%) cases of acute myeloid leukaemia (AML), 2 out of 10 (20 %) cases of chronic myeloid leukaemia (CML), 0 out of 29 cases of lymphoid leukaemia (ALL + CLL), and 1 out of 104 normal humans had decreased MPO scores. These figures correlated well with the more simple counting of PMN lacking MPO in the same groups: 8 out of 21 (37 %) cases of AML, 6 out of 10 (60 %) cases of CML and 0 out of 29 cases of lymphoid leukaemia showing more than 4 % PMN lacking MPO. In cases of otherwise unclassifiable acute leukaemia, a decreased MPO score and an increased number of MPO‐deficient PMN suggests the diagnosis of AML and not ALL. Counting the number of PMN lacking MPO was found to be a time‐saving and even more reliable method than the semiquantitative scoring of MPO activity in PMN.