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Soluble I 131 fibrin form high molecular weight complexes in prothrombin‐depleted, recalcified plasma at +37 °C
Author(s) -
BROSSTAD FRANK,
KIERULF PETER
Publication year - 1983
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1983.tb02162.x
Subject(s) - fibrin , chemistry , fibrinogen , size exclusion chromatography , calcium , thrombin , chromatography , biochemistry , organic chemistry , immunology , enzyme , platelet , biology
Gel filtration of plasma samples containing I 125 fibrinogen and either I 131 des‐AA soluble fibrin or I 131 des‐AABB soluble fibrin, were performed on Sepharose 6B C1 columns at + 37 °C, equilibrated and developed with prothrombin‐depleted plasma, either recalcified or containing EDTA. With EDTA, des‐AA fibrin eluted in a homogenous, monomeric form, while des‐AABB fibrin had an elution profile indicating high molecular weight derivatives. In the presence of calcium ions, both des‐AA and des‐AABB soluble fibrin eluted mainly as high molecular weight derivatives. It could not, however, be decided whether the high molecular weight derivatives consisted of fibrin molecules alone, or of co‐polymers of fibrin‐fibrinogen. It is concluded that soluble des‐AA fibrin and soluble des‐AABB fibrin in plasma at + 37 °C and in the presence of calcium ions, participate in the formation of high molecular weight derivatives of fibrin.