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Lyophilized, reconstituted plasma as substrate for thrombin activity assays
Author(s) -
BROSSTAD F.,
ELDE A.T.,
GODAL H.C.
Publication year - 1983
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1983.tb02161.x
Subject(s) - thrombin , substrate (aquarium) , chemistry , human plasma , chromatography , platelet poor plasma , fibrinogen , context (archaeology) , plasma , biochemistry , platelet , immunology , biology , ecology , paleontology , physics , quantum mechanics
Due to more precise readability of the visual gel point, citrated pooled plasma is a better substrate than is purified fibrinogen for thrombin activity assays. Since a lyophilized plasma would be more practical in this context, we have studied whether citrated plasma can be lyophilized and reconstituted without jeopardizing its properties as a thrombin‐assay substrate. While undialysed, lyophilized/reconstituted plasma proved unsuccessful due to a considerable pH shift, the clotting properties of pooled human citrated plasma could be fully preserved if the plasma prior to freeze‐drying/reconstitution was dialysed against a citrate‐NaCl‐diemal buffer pH 7.4 Such plasma could be kept at room temperature for at least 6 weeks or at –20 °C for 18 months and could, subsequent to reconstitution, demonstrate thrombin substrate properties identical to those of freshly prepared plasma.