Premium
Terminal Deoxynucleotidyl Transferase (TdT) in Human Foetuses
Author(s) -
Bonati Antonio,
Casoli Claudio,
Starcich Bruno,
Buscaglia Mauro
Publication year - 1983
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1983.tb01541.x
Subject(s) - terminal deoxynucleotidyl transferase , bone marrow , cord blood , pathology , human bone , fetus , biology , andrology , medicine , microbiology and biotechnology , tunel assay , immunology , immunohistochemistry , pregnancy , in vitro , biochemistry , genetics
The liver, bone marrow and cord blood of 30 human foetuses between the 15th and 21st weeks of gestational age were examined. Liver suspensions were investigated both by immunofluorescent and biochemical assay; cord blood suspensions and bone marrow touches with immunofluorescent assay. The results indicate the existence of a few TdT+ cells in the liver, cord blood and bone marrow of human foetuses in the ages studied. The peaks for each organ were: 15 + 1.60 (mean + SD) TdT+ cells in the liver, 12 + 2 in the bone marrow and 18 + 2.83 in the cord blood. In all the organs TdT+ cells decrease in the later weeks. In the liver, the results of the immunofluorescent assay were confirmed by the biochemical activity.