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In Vitro Clonogenic Assays in Selective Neutropenia
Author(s) -
Brown R. D.,
Yuen E.,
Kronenberg H.,
Rickard K. A.
Publication year - 1983
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1983.tb01452.x
Subject(s) - neutropenia , clonogenic assay , medicine , granulocyte , immunology , myeloid , in vitro , monocyte , gastroenterology , biology , chemotherapy , biochemistry
Granulocyte‐monocyte colony forming cell (GM‐CFC) concentration and the proportion of GM‐CFC in DNA synthesis (S) were determined in 43 patients with varying degrees of selective neutropenia, including 5 patients who were normal extremes (2.0–2.5 times; 10 9 neutrophils/1), to study the diagnostic and prognostic significance of clonogenic assays and to determine the response of the committed myeloid stem cell to neutropenia. The proportion of GM‐CFC in S proved to be a more useful parameter than the GM‐CFC concentration. 75% of the patients with greater than 60% GM‐CFC in S returned to normal within 1 month. Patients with less than 20% GM‐CFC in S had at least a 10 times greater incidence of developing a malignant or autoimmune disease than the other 28 patients. The 11 patients with 41–51% in GM‐CFC in S had greater than 1 times; 10 9 neutrophils/1 and no significant clinical problems but all have remained mildly neutropenic for a long period. An indirect relationship existed between the degree of neutropenia and the proportion of GM‐CFC in S (r = ‐0.70).