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Platelet BTG Release in Vitro Induced by Mechanical and Chemical Stimulus: Correlation with the Aggregation Curve Parameters
Author(s) -
Santos M. T.,
Valles J.,
Aznar J.,
Villa P.
Publication year - 1982
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1982.tb00610.x
Subject(s) - platelet , thrombin , chemistry , aspirin , stimulation , platelet activation , platelet aggregation , in vivo , in vitro , ingestion , platelet rich plasma , biophysics , beta thromboglobulin , biochemistry , medicine , biology , microbiology and biotechnology
The in vitro release of B‐thromboglobulin (BTG) from platelets after stirring the platelet rich plasma (PRP) 5 min (37°C/1000rpm) was studied in a group of 13 healthy subjects before and after ‘in vivo’ aspirin ingestion, 1 g/d for 3 d. A significant reduction in the platelet BTG release (31%) was observed after aspirin ingestion in comparison to the pre‐aspirin values, suggesting that the BTG release by stirring is related to a platelet activation process. Small doses of collagen (0.625 μg/ml) and thrombin (0.1 U/ml) always released platelet BTG, whether or not there was a visible platelet aggregation, suggesting that platelet activation is not necessarily followed by visible platelet aggregation. A significant correlation was found between the BTG released by stimulation with collagen (2.5 μg/ml) and thrombin (0.2 U/ml) and the maximum velocity and intensity of the aggregation curve parameters.