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Effect of Blood Derived Monocytes on the Promotion of in Vitro Erythropoietic Colony Growth in Human Bone Marrow Cultures
Author(s) -
GRILLI GUALTIERO,
CARBONELL FELIX
Publication year - 1982
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1982.tb00605.x
Subject(s) - peripheral blood mononuclear cell , monocyte , bone marrow , ficoll , in vitro , immunology , chemistry , andrology , microbiology and biotechnology , biology , medicine , biochemistry
The effect of monocytes on the promotion of erythropoietic burst formation in human bone marrow methylcellulose cultures was studied in a burst‐promoting activity (BPA)‐poor system. Irradiated Ficoll‐Isopaque separated blood mononuclear cells stimulated BFU‐E growth proportionally to the number of irradiated cells added. Monocyte‐depleted mononuclear cells stimulated as well as total mononuclear cells. Monocyte‐concentrates stimulated when added at low concentrations. Monocyte‐depleted mononuclear cells, although stimulatory at higher concentrations, did not stimulate at the concentrations used for monocyte‐concentrates.