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Surface Antigens on Normal and Leukaemic Human Cells Detected by Monoclonal Antibodies
Author(s) -
Morstyn George,
Metcalf Donald,
Burgess Antony,
Fabre John W.
Publication year - 1981
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1981.tb01619.x
Subject(s) - monoclonal antibody , antigen , eosinophil , myeloid , granulocyte , antibody , microbiology and biotechnology , biology , immunology , progenitor cell , stem cell , asthma
Surface antigens were analyzed on normal human marrow and chronic myeloid leukaemic cells using 4 monoclonal mouse anti‐human antibodies. The fluorescence‐activated cell sorter was used to quantify the binding of each antibody to different subpopulations of cells, and sorted fractions were cultured in agar‐medium to assay for granulocyte‐macrophage and eosinophil precursors. All cells in the granulocyte series including colony‐forming cells bound a similar quantity of an antibody to the human leucocyte common antigen. This antibody did not bind to cells in the erythroid series. A monoclonal antibody to antigen present on brain, lymphocytes and granulocytes (and almost certainly homologous to the W3/13 antigen of the rat) bound to the cells in the order: blast > promyelocytes and myelocytes > granulocytes. The third monoclonal antibody was directed against a determinant of the leucocyte common antigen present predominantly on B lymphocytes and absent from the myeloid series. The fourth antibody, directed against the human homologue of Thy‐1, reacted with less than 1% of marrow cells, none of which appeared to be granulocyte or eosinophil progenitors. The leucocyte common antigen and the brain‐lymphocyte‐granulocyte‐antigen were also present on colony‐ and cluster‐forming cells from a patient with chronic myeloid leukaemia. Using the low angle and wide angle light scatter properties of CML blood cells, 7‐fold enrichment was obtained for progenitor cells from chronic myeloid leukaemia. With the monoclonal antibodies up to 4‐fold enrichment was obtained.