Premium
Platelet Lesion of Collection
Author(s) -
Mccue John P.,
Stevens David J.,
Kermon Vivian L.,
Ashe Michol C. S.,
Heim Lyle R.
Publication year - 1981
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1981.tb01407.x
Subject(s) - platelet , adenosine diphosphate , epinephrine , anticoagulant , whole blood , chemistry , organelle , lesion , andrology , platelet aggregation , pharmacology , medicine , biochemistry , pathology
In the early stages of blood collection for transfusion, whole blood is subjected to trauma from the anticoagulant. The blood – anticoagulant mixture does not attain a pH at which platelets can remain functional (approximately pH 6.0) until about 25% of the unit has been collected. We have examined platelet ultrastructure and in vitro aggregation responses to adenosine diphosphate (ADP) and epinephrine (EPN), to assess the platelet trauma of collection into citrate‐phosphate‐dextrose. For comparison blood was collected by 2 methods: one was conventional (CC), the other metered anticoagulant into the blood as it was collected (MC). Platelets from CC blood showed disruption of organelles and depletion of the dense bodies, whereas platelets from MC blood showed no ultrastructural damage. In addition, biphasic aggregation in response to ADP or EPN was seen in MC platelets but not in CC platelets.