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Different Types of Endotoxin‐Induced Release of Colony Stimulating Factors by Adherent Leucocytes in the Presence of Fresh and Heat‐Inactivated Autologous Serum
Author(s) -
Hinterberger Wolfgang,
Mittermayer Karl,
Paukovits Walter,
Singer Jack
Publication year - 1981
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1981.tb01392.x
Subject(s) - granulopoiesis , colony stimulating factor , in vitro , lipopolysaccharide , chemistry , sephadex , microbiology and biotechnology , immunology , andrology , stem cell , haematopoiesis , biology , biochemistry , medicine , enzyme
Human adherent leucocytes stimulate in vitro granulopoiesis by releasing colony stimulating factors (CSF), which promote the growth of myeloid committed stem cells (CFU c ). The effect of lipopolysaccharide (LPS) on CSF generation by adherent leucocytes was studied with fresh and heat‐inactivated autologous serum. Adherent leucocyte conditioned media were fractionated on Sephadex G‐75. LPS, in the presence of fresh serum, caused a significant increase of CSF release by adherent leucocytes within 1 h. Heat inactivation of autologous serum abolished this effect. Adherent leucocyte CSF had 3 activity peaks at > 75000, 23000 and < 4000 daltons. LPS‐fresh serum initiated CSF, harvested after culture periods of 1 and 24 h, disclosed identical elution profiles.