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Expression of Common Acute Lymphoblastic Leukaemia Antigen and Terminal Deoxynucleotidyl Transferase in Normal Mononuclear Blood Cells during Diffusion Chamber Culture
Author(s) -
Jäger Gundula,
Lau Barbara
Publication year - 1981
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1981.tb00444.x
Subject(s) - terminal deoxynucleotidyl transferase , peripheral blood mononuclear cell , antigen , population , microbiology and biotechnology , biology , pathology , immunology , chemistry , tunel assay , in vitro , immunohistochemistry , medicine , genetics , environmental health
Peripheral mononuclear blood cells of 2 healthy individuals were cultured over a 13 d period in diffusion chambers. A high percentage of cALL antigen positive cells and later on TdT containing cells appeared during culture. The cALL + cells could be clearly distinguished into 2 groups: a population with weak and diffuse fluorescence and a small number of ring‐like brightly dyed cells. The first group develops into TdT + cells and therefore resembles immature T‐cells. The interpretation of the ring‐like stained cells is still preliminary, but it cannot be excluded, that these cells represent a cell population with more than lymphatic differentiation capacity.