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Proliferation and Differentiation in Diffusion Chambers of Marrow, Blood and Spleen Cells from Patients with Chronic Myeloid Leukaemia during Chronic Phase and Blastic Transformation
Author(s) -
Nilsson Bo,
Olofsson Eva,
Olofsson Tor,
Olsson Inge
Publication year - 1980
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1980.tb02753.x
Subject(s) - spleen , bone marrow , chronic myeloid leukaemia , myeloid , immunology , peritoneal cavity , medicine , pathology , cancer research , biology , anatomy
The proliferative and differentiating capacity was compared between immature normal marrow cells and marrow, blood or spleen cells from patients with chronic myeloid leukaemia (CML) in chronic and blastic phases. Low density cells highly enriched in myeloblasts and promyelocytes were cultured in diffusion chambers (DC) and implanted into the peritoneal cavity of mice pretreasted with cyclophosphamide. In CML of chronic phase cell production was higher than normal with the exception of spleen cells. Total cell production was lower in blastic phase than in CML chronic phase. The [ 3 H]‐TdR labelling index of myeloblasts of blastic phase CML increased considerably upon implantation in DC consistent with re‐entry into active proliferation. Immature normal cells give rise mostly to macrophages while the corresponding CML cells of chronic phase give a much higher PMN production with peaks after 12–18 d. Also in CML of blastic crisis the blasts differentiate into mature PMNs, but the maturation time is shortened with peaks of PMNs after 5–10 d. DC cultures of spleen cells from patients with CML showed a less differentiating capacity with a very low PMN production compared to cultures of marrow or blood cells. Our results indicate intrinsic differences in growth and maturation capacity between normal immature granulopoietic cells and cells from patients with CML of chronic phase or blastic crisis. The results may also indicate intrinsic differences between spleen, blood or marrow cells in CML.