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Myelopoietic Stem Cells (CFUc) in the Blood and Bone Marrow of Children with Acute Lymphoblastic Leukaemia and Lymphosarcoma, Cultivated without an Exogenous Supply of Colony Stimulating Factor (CSF)
Author(s) -
Prindull G.,
Meulen N. V. D.,
Jentsch E.,
Prindull B.,
Schröter W.
Publication year - 1980
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1980.tb01587.x
Subject(s) - bone marrow , medicine , haematopoiesis , stem cell , immunology , biology , genetics
26 children with acute lymphoblastic leukaemia (ALL) and 7 additional patients with lymphosarcoma in leukaemic transformation (LSA) have been studied with respect to the content of myelopoietic stem cell (CFUc) in blood and bone marrow. The methylcellulose culture technique (Iscove et al 1974) was employed in the absence of an exogenous source of colony stimulating factor (CSF). During active disease, CFUc colony formation was absent from patients with ALL, but was present in 2 patients with LSA. 2 therapeutic regimens were employed. Colony formation from bone marrow CFUc was highly variable during remission maintained by either regimen, with no clear relation to clinical stage, number of monocytes or circulating neutrophils. Patients with LSA consistently had high numbers of bone marrow CFUc. CFUc were low or absent from the blood. In conclusion, CFUc are absent from the bone marrow in active ALL, but may be present in active LSA. For the purpose of monitoring children with ALL during therapy, determination of blood or bone marrow CFUc was not found in this study to be helpful.