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Normal and Leukaemic Human Haemopoietic Cells in Diffusion Chamber
Author(s) -
Laurent Monique,
ClémanceyMarcille Geneviève,
Hollard Daniel
Publication year - 1980
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1980.tb01328.x
Subject(s) - progenitor cell , haematopoiesis , bone marrow , in vitro , population , precursor cell , biology , microbiology and biotechnology , peripheral blood , andrology , immunology , chemistry , stem cell , biochemistry , medicine , environmental health
Leukaemic human bone marrow and peripheral blood cells were cultured for 25 d in diffusion chambers implanted into cyclophosphamide treated mice. Normal bone marrow cells were cultured simultaneously. These cells were studied both morphologically and functionally (CFU‐C). The leukaemic cells behaved heterogeneously, 2 groups being distinguishable in accordance with their initial in vitro growth pattern (1: no growth or microcluster growth, 2: macrocluster growth). Group I showed progressive cellular death with a diminution of granulocytic progenitors and the appearance of a predominantly macrophagic population. This behaviour resembled that of the control group. The initial microcluster growth pattern remained identical throughout the entire culture period. Group 2, after considerable cellular death up to d 5, showed an explosive proliferation of the granulocytic progenitors and incomplete differentiation (up to myelocyte). The initial macrocluster growth pattern remained identical.