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Activation of the Bactericidal Capacity of Polymorphonuclear Granulocytes after Surgery, Measured with a New in vitro Assay
Author(s) -
Palmblad J.
Publication year - 1979
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1979.tb02846.x
Subject(s) - granulocyte , in vitro , bacteria , microbiology and biotechnology , phagocytosis , chemistry , immunology , medicine , biology , biochemistry , genetics
A classic in vitro polymorphonuclear (PMN) granulocyte bactericidal system was used alongside a newly developed modification to see whether the new assay would increase the possibility to detect a stimulation of PMN bactericidal functions. In the new assay each granulocyte was provided with 30–40 bacteria, which is quite close to the maximal killing capacity (usually 60 bacteria per PMN). Granulocytes were obtained from 8 patients the day before, the day after and 2 d after they underwent thoracotomy with cardiopulmonary by‐pass (CPS). The granulocytes from all patients showed an increased capacity to kill Staph. aureus in vitro 2 d after the operation, compared to before, when the submaximal bacterial concentration per granulocyte was used, whereas no change was observed with the standard bacterial concentration (3–4 bacteria per granulocyte). Thus, the new assay might make it possible to observe an enhanced PMN bactericidal ability.