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Erythrocyte and Leucocyte Enzymes in a Case of Paroxysmal Nocturnal Haemoglobinuria
Author(s) -
Hanel H. K.,
Mejer J.,
Mørck H. I.
Publication year - 1979
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1979.tb02805.x
Subject(s) - purine nucleoside phosphorylase , adenosine deaminase , enzyme , biochemistry , chemistry , hypoxanthine phosphoribosyltransferase , pyruvate kinase , adenosine , adenosine kinase , hypoxanthine guanine phosphoribosyltransferase , purine , mutant , glycolysis , gene
In a patient with paroxysmal nocturnal haemoglobinuria (PNH) enzymatic activities of erythrocytes and leucocytes were studied. Studies of autohaemolysis were also performed. The following erythrocytary enzymes were measured: Glucose‐6‐phosphate dehydro‐genase (G‐6‐PD), pyruvate kinase (PK), glutathione reductase (GR), and acetyl‐cholinesterase (AcChE). The following enzymes were measured in leucocytes: Adenosine deaminase, purine nucleoside phosphorylase, adenine phosphoribosyltransferase, hypoxanthine phosphoribosyltransferase and adenosine kinase. Normal activity of G‐6‐PD, GR and PK in erythrocytes was found. In leucocytes and lymphocytes activity of purine nucleoside phosphorylase was reduced. Autohaemolysis in vitro was increased, which could not be compensated by addition of glucose or ATP.