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Evaluation of Phagocytic and Bactericidal Activities of Neutrophil Granulocytes
Author(s) -
Braconier J. H.,
Odeberg H.
Publication year - 1979
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1979.tb02741.x
Subject(s) - phagocytosis , lysis , bacteria , extracellular , intracellular , microbiology and biotechnology , granulocyte , thymidine , tonicity , bacterial cell structure , neutrophil extracellular traps , biology , opsonin , chemistry , biochemistry , immunology , inflammation , in vitro , genetics
A method for evaluation of human neutrophil granulocyte function based on the combined determination of total and extracellular bacteria is described. The total number of surviving bacteria is assessed by the determination of colony forming units (CFU) after hypotonic lysis of granulocytes. Extracellular viable bacteria can be determined by the incorporation of 14 C‐leucine or 3 H‐thymidine into bacterial macromolecules since there is a linear relationship between macromolecular synthesis and bacterial number and insignificant amounts of both 14 C‐leucine and 3 H‐thymidine is taken up by intracellular viable organisms. This method might be suitable for the differentiation of defects in phagocytosis and intracellular killing of various bacteria.