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Labelling of Human and Rabbit Platelets with 111 Indium‐Oxine Complex
Author(s) -
Schmidt Kai Gjerløff,
Rasmussen Jens Wæver
Publication year - 1979
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1979.tb02679.x
Subject(s) - labelling , platelet , centrifugation , chemistry , in vivo , chromatography , yield (engineering) , biochemistry , immunology , biology , materials science , microbiology and biotechnology , metallurgy
Platelets suspended in buffer were labelled with 111 Indium‐oxine. In the isolation procedure the platelets and a fraction of the erythrocytes are processed simultaneously, whereby the erythrocytes serve as a supporting cushion in the centrifugation steps. Various factors which influence the labelling effectiveness were examined, and the method's utility was estimated by in viva studies in rabbits. A platelet concentration of more than 800 times 10 9 /l and an oxine concentration of approximately 6 μg/ml in a plasma‐poor system (transfer of platelets from plasma to buffer without further washing steps) will yield a high labelling efficiency. If the platelet isolation yield is to be large, a slight erythrocyte‐leucocyte contamination must be accepted. Our experimental results indicated that this is permissible. In vivo investigations of 5 rabbits showed in vivo recoveries of platelets from 61–74% and mean survival times from 69h to 110h. This supports the assessment that the method of platelet isolation and labelling is gentle.