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Cell Proliferation and DNA Dependent DNA Polymerase Estimation in Acute Lymphoblastic Leukaemia during Treatment with Prednisone and Vincristine
Author(s) -
Wantzin Gunhild Lange
Publication year - 1979
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1979.tb00435.x
Subject(s) - lymphoblast , dna synthesis , vincristine , microbiology and biotechnology , cell cycle , dna polymerase , thymidine , prednisone , primer (cosmetics) , dna , mitotic index , mitosis , cell growth , acute lymphocytic leukemia , biology , chemistry , cell , cell culture , medicine , immunology , biochemistry , leukemia , chemotherapy , lymphoblastic leukemia , genetics , organic chemistry , cyclophosphamide
The presence of DNA polymerase and primer‐template DNA in lymphoblast nuclei by measuring the in vitro incorporation of 3 H‐thymidine‐5′‐triphosphate ( 3 H‐TTP) was studied in 10 patients with acute lymphoblastic leukaemia. Protein synthesis and various other cytokinetic parameters were also studied. After prednisone (P) administration a marked decrease in 3 H‐TTP labelling index ( 3 H‐TTP LI) was apparent together with an inhibition of 3 H‐leucine incorporation ( 3 H‐LEU LI) into lymphoblasts. A moderate decrease in 3 H‐TDR labelling index ( 3 H‐TDR LI) and a later decrease in mitotic index (MI) were seen. Single cell DNA measurements showed a depletion of 3 H‐TDR labelled lymphoblasts in early part of S‐phase apparent at 24 h lasting up to 54 h after P administration. Vincristine given as a flash injection later in the study period caused an immediate rise of the MI, at the same time the P induced decline in 3 H‐TTP LI, 3 H‐TDR LI and 3 H‐LEU LI were continued in most patients. P is thought to damage the cells both in and outside the cell cycle. In the cell cycle the effect of P is an arresting effect in G 1 .