Premium
Cytochemistry of Marrow and Extramedullary Adipocytes in Monolayer Cultures
Author(s) -
Tavassoli Mehdi
Publication year - 1978
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1978.tb02464.x
Subject(s) - adipose tissue , cytochemistry , adipocyte , esterase , bone marrow , fibroblast , chemistry , cell , white adipose tissue , pathology , biology , microbiology and biotechnology , biochemistry , enzyme , immunology , in vitro , medicine
The behavior of isolated, disaggregated white adipose cells obtained from marrow and extramedullary sites in the rabbit was compared in monolayer cultures. Both cell types transform within 2 weeks into fibroblast‐like cells which can be maintained in subcultures. Both in freshly isolated state and in fibroblast‐like state the marrow adipocyte contains esterase activities reacting with naphthol AS acetate and D‐chlor‐acetate. These activities are not present in extramedullary cells. In addition, α‐naphthyl butyrate‐reacting esterase, present in both cell types, is fluoride‐resistant in marrow adipocytes but labile in extramedullary cells. These differences are consistent with heterogeneity of white adipose tissue and suggest different potentials for marrow and extramedullary adipocytes.