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ATP‐ase Activity of Purified Human Normal T‐ and B‐Lymphocytes
Author(s) -
Kragballe Knud,
Ellegaard Jørgen
Publication year - 1978
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1978.tb02457.x
Subject(s) - microbiology and biotechnology , chemistry , biochemistry , biology
In 15 healthy individuals the ATP‐ase activity of circulating B‐lymphocytes was found to be significantly higher than of T‐lymphocytes. Two different methods were used for the purification of B‐ and T‐lymphocytes and the result was independent of which one of these methods was used. The demonstration of different ATP‐ase activities of un‐stimulated B‐ and T‐lymphocytes corresponds well with previously published histo‐chemical findings in lymphatic tissues examined for ATP‐ase activity. It is concluded from the present study that because of overlapping values determination of the lymphocyte ATP‐ase activity cannot serve as a single marker for the B‐ and T‐cell nature of human lymphocytes, but demonstration of an unusual high ATP‐ase activity in unstimulated lymphocytes will serve as additional identification of these as belonging to the B‐lymphocyte population.