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Activation of Complement by Stroma from Normal and Paroxysmal Nocturnal Haemoglobinuria Red Cells
Author(s) -
Sandre G.,
Corrocher R.,
Falezza G. C.,
Aprili G.,
Caffi S.
Publication year - 1978
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1978.tb02456.x
Subject(s) - stroma , lysis , haemolysis , alternative complement pathway , hemolysis , chemistry , complement system , classical complement pathway , cytolysis , sephadex , biochemistry , biology , antibody , immunology , enzyme , in vitro , immunohistochemistry , cytotoxic t cell
Stroma from normal, AET‐treated and PNH red cells and their KCl‐extracts (partially purified on Sephadex G‐200) are able to trigger the activation of the alternative complement pathway. This fact has been demonstrated by:1 — the lysis of PNH cells incubated in serum treated with stroma from normal or PNH‐RBC or with their extracts; the addition of Mg 2+ or Ca 2+ or of their chela‐tors (EDTA, EGTA) to the extract‐treated serum enhances or abolishes the lysis 2 — the reduction of complement activity in fresh serum incubated for 60' with PNH‐ extract 3 — the appearance of C 3 breakdown products in serum incubated with PNH‐extract, demonstrated by crossed immunoelectrophoresis.In contrast, the same stroma (or extract) inhibits the sucrose lysis test, in which the lysis takes place through the classical complement pathway. No differences on the complement activation were observed between PNH and normal RBC stroma and between their chromatographic extracts. These findings may suggest the possible role of diurnal variation of Mg 2+ and Ca 2+ concentration in precipitating haemolytic attacks and the possibility that small amount of circulating red cell stroma might maintain the haemolysis of PNH RBC.