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Inhibition of Haemopoietic Stem Cell Proliferation by a Diffusible Product of Bone Marrow Cells
Author(s) -
Benestad H. B.,
Testa N. G.,
Lajtha L. G.
Publication year - 1978
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1978.tb01549.x
Subject(s) - bone marrow , microbiology and biotechnology , haematopoiesis , cell growth , cell , cell cycle , stem cell , biology , growth factor , spleen , granulocyte , colony stimulating factor , immunology , chemistry , biochemistry , receptor
In a double diffusion chamber (DC) culture system bone marrow cells elaborated diffusible factor(s) that prevented spleen colony‐forming cells (CFU‐S), but not PHA stimulated lymphocytes, from entering cell cycle. Mature granulocytes and macrophages did not produce such factor(s). Various numbers of steady‐state or regenerating mouse bone marrow cells were cultured in single diffusion chambers for periods up to 7 d. After the initial cell loss, the net growth of CFU‐S was inversely related to both total cell number and CFU‐S number in DC. Diffusible factors rather than cell‐to‐cell contact appeared to be involved in the inhibition, even though we were not able to demonstrate inhibition of net growth of CFU‐S with the double chamber approach.