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Inhibition of Myeloid Differentiation by Mature Granulocytes can be Reversed by Humoral Factors
Author(s) -
Løvhaug Dagfinn
Publication year - 1978
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1978.tb00387.x
Subject(s) - granulocyte , bone marrow , myeloid , granulocyte colony stimulating factor receptor , myelopoiesis , immunology , biology , haematopoiesis , microbiology and biotechnology , cell growth , chemistry , andrology , stem cell , biochemistry , medicine
Human mature granulocytes added to in vivo diffusion chamber (DC) cultures of murine bone marrow cells (BMC) led to a late reduction of CFU‐C, day 5, and proliferative granulocytes, day 6. Cell free DC fluid harvested on day 4 from chambers with BMC and mature granulocytes slightly reduced granulocyte formation in new DC cultures, but not more than DC fluid from control cultures (BMC). Mice which had carried chambers of either group for 4 d, were not different when used as hosts for new DC cultures. However, they were less able to stimulate cell growth in DC than untreated mice. Transfer of DC to new host mice on day 2 completely reversed the inhibition by mature granulocytes which otherwise could be detected in numbers of CFU‐C on day 5 and proliferative granulocytes on day 7. However, CFU‐S numbers were reduced on day 5 following reimplantation indicating an increased differentiation of CFU‐S. Transfer of DC as late as day 4, rapidly restored the granulocyte numbers in the granulocyte co‐culture group suggesting that also more differentiated granulocytes were stimulated to further growth and differentiation. It is proposed that factors from mature granulocytes reversibly inhibit myeloid differentiation. Differentiation can again be induced by factors which reach the DC shortly after intraperitoneal implantation in a mouse.