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The Effect of Phospholipase C on Rat Blood Platelets in Vivo
Author(s) -
Giercksky KarlErik
Publication year - 1977
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1977.tb02333.x
Subject(s) - in vivo , platelet , phospholipase c , chemistry , pharmacology , medicine , biology , biochemistry , enzyme , genetics
Rat blood platelets were treated with phospholipase C in vitro or phospholipase C was injected i.v. to rats. In both cases its effect on the functions of the platelets in vivo has been studied. No change was found in primary bleeding time or in platelet survival. Treatment with phospholipase C gave a moderate reduction of ADP‐induced platelet aggregation in the pulmonary circulation whereas the aggregation induced by thrombin was unchanged. I.v. injection of phospholipase C caused a rapid, very moderate and transient increase of 51 Cr‐activity in the lungs without concomitant overt respiratory distress. A moderate increase in 51 Cr‐activity was noted in liver and kidney 24 and 48 h after injection of phospholipase C. This may be caused by a slightly increased leakage of 51 Cr‐labelled material from the platelets during exposure to phospholipase C.