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Discrimination of B, T and Null Lymphocytes by Esterase Cytochemistry
Author(s) -
Higgy K. E.,
Burns G. F.,
Hayhoe F. G. J.
Publication year - 1977
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1977.tb02098.x
Subject(s) - cytochemistry , esterase
A technique has been devised which optimally demonstrates non‐specific esterase activity in human blood lymphocytes. The reaction is carried out on smears fixed with formalin vapour, using α‐naphthol butyrate as substrate at pH 8 and at a low concentration for a short incubation period. The pattern of esterase activity revealed by this method provides a discriminating marker for mature T‐lymphocytes, which show dense, localised, dot‐like positivity, and a probable marker for ‘null’ cells in the form of scattered granular positivity. B cells appear to be negative. Monocytes show a clearly different pattern of granular positivity.