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Increased ATP‐ase Activity of Lymphocytes after Rosette Formation with Sheep Red Blood Cells
Author(s) -
Kragballe Knud,
Ellegaard Jørgen
Publication year - 1977
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1977.tb02091.x
Subject(s) - rosette formation , neuraminidase , rosette (schizont appearance) , biology , lymphocyte , microbiology and biotechnology , receptor , immunology , chemistry , biochemistry , virus
In a number of experiments it was demonstrated that in the E‐rosette assay for human T‐lymphocytes using sheep red blood cells, the ATP‐ase activity of the lymphocytes increased in proportion to the number of rosettes being formed. Also, after increasing the number of rosettes by treatment of the sheep red blood cells (SRBC) with neuraminidase and after diminishing the number of rosettes by treatment of the lymphocytes with antilymphocyte globulin (ALG), change in the ATP‐ase activity was proportional to alterations in the number of rosettes being formed. ALG itself stimulated the lymphocyte ATP‐ase activity, possibly through activation of the same receptor sites as used by SRBC.