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Preparation of Concentrated Platelet Suspensions by Dehydration against Polyethyleneglycol 20,000
Author(s) -
Akkerman J. W. N.,
Gorter G.,
Wester J.,
Sixma J. J.
Publication year - 1976
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1976.tb02843.x
Subject(s) - dehydration , platelet , chemistry , chromatography , dialysis , suspension (topology) , platelet rich plasma , tonicity , biochemistry , surgery , medicine , mathematics , homotopy , pure mathematics
Gel filtered platelet suspensions were concentrated by repeated dehydration against polyethyleneglycol 20,000 followed by dialysis against slightly hypotonic buffer. This method increased the platelet concentration 2–3 times with a recovery of 80–100%. The final cell suspension closely resembled the original platelet rich plasma as tested by a number of platelet tests.