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Protection of Rats by Phospholipase C from Bacillus cereus against the Effects of Intravenous Infusions of Purified Tissue Thromboplastin
Author(s) -
GIERCKSKY KARLERIK,
BJØRKLID EIRIK
Publication year - 1976
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1976.tb01189.x
Subject(s) - thromboplastin , tissue factor , platelet , pharmacology , partial thromboplastin time , chemistry , phospholipase , disseminated intravascular coagulation , fibrin , coagulation , phospholipase c , bolus (digestion) , biochemistry , medicine , enzyme , immunology
Infusions of purified tissue thromboplastin in rats cause the accumulation of fibrin and platelets in the lungs and produce marked changes in the platelet count and in the coagulation factors V, VII and VIII. Tissue thromboplastin in a dose corresponding to less than 2 μg of protein per rat is lethal when given as a bolus injection. Simultaneous i.v. administration of purified phospholipase C effectively prevents all these changes and protects rats from otherwise lethal doses of tissue thromboplastin. The necessary doses of phospholipase C are well below the toxic level for phospholipase C alone.