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μ‐Chain Disease in a Case of Chronic Lymphocytic Leukaemia and Malignant Histiocytoma
Author(s) -
Axelsen N. H.,
Harboe M.,
Jønsson V.,
Videbæk AA.
Publication year - 1976
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1976.tb01141.x
Subject(s) - medicine , chronic lymphocytic leukemia , disease , pathology , dermatology , leukemia , immunology
Agarose gel electrophoresis of serum from the eleventh case of μ‐chain disease (μ‐CD) showed two distinct abnormal bands due to free light chains and k ‐complexes with other serum proteins. This caused diagnostic difficulties on conventional immunoelectrophoresis, and crossed immunoelectrophoresis with intermediate gel is demonstrated as a superior tool for investigation of μ‐CD. A simple rocket Immunoelectrophoresis method for μ‐CD screening is also shown. The μ‐CD protein had α‐2‐mobility and a part of the protein carried k ‐determinants probably due to secondary binding. Preparative ultracentrifugation and size chromatography on serum demonstrated that the μ‐CD protein was present in serum in several sizes, the smallest being a dimer of μ‐chains. Due to scarcity of material the abnormal protein could not be characterized further.