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The Failure of Granulocytes to Produce Transcobalamin I (TC I)
Author(s) -
Hall Charles A.
Publication year - 1976
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1976.tb01135.x
Subject(s) - granulocyte , isoelectric focusing , lysis , chemistry , biochemistry , immunology , microbiology and biotechnology , biology , enzyme
The hypothesis that transcobalamin I (TC I) originates from granulocytes was tested by comparing the isoelectric focusing (IEF) patterns of the R‐type binder (cobalophilin) of vitamin B 12 of: 1) lysed granulocytes, 2) 24 h granulocyte output, and 3) plasma. The preparations came from the blood of 5 normal subjects and 4 with myeloproliferative states. The cobalophilin released into a culture of granulocytes resembled the binder in the granulocytes initially. TC I, defined as the α 1 cobalophilin with components isoelectric between pH 2.9‐3.35 and as a carrier of native plasma B 12 , was not released by granulocytes. The granulocyte binder of leukaemic granulocytes did not differ from the normal in content per cell or amount released.