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Human Granulocytopoietic Colonies in Diffusion Chambers in Mice: Growth of Colonies and the Effect of Host Irradiation
Author(s) -
Jacobsen Niels,
Fauerholdt Lis
Publication year - 1976
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1976.tb01124.x
Subject(s) - irradiation , biology , host (biology) , colony forming unit , fibrin , andrology , bone marrow , microbiology and biotechnology , centrifugation , cell , diffusion , immunology , chemistry , biochemistry , genetics , bacteria , medicine , physics , nuclear physics , thermodynamics
Normal human non‐separated bone marrow cells were cultured in fibrin clots in diffusion chambers implanted intraperitoneally in mice, and harvested at different intervals by a previously described chamber centrifugation technique. This method demonstrates the presence of cell aggregates in the diffusion chambers. When the chambers are implanted in irradiated mice (450 R) and retransplantated into newly irradiated mice every seventh day, a continous increase in number of cells per granulocytopoietic aggregate is observed from day 8 to day 21. This is compatible with the view that the aggregates are colonies. The term ‘colony forming unit diffusion chamber’ (CFUD) is suggested to denote the ancestor(s) of the colonies. However, formal proof that one colony is derived from one cell is lacking. Preirradiation of mice with 450 R significantly increases the number of neutrophilic granulocytopoietic colonies at day 14, provided the chambers are retransplantated to newly irradiated mice at day 7, indicating that the neutrophilic colony forming unit or its progeny is involved as at least one of the targets of the stimulating effect of host irradiation. In contrast, no effect of host irradiation on the numbem of eosinophilic colonies was observed. Aggregates of megakaryocytic cells were present during the entire culture period.