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Selective Eradication of Rat Bone Marrow Erythroid Cells by Administration of Cytosine Arabinoside
Author(s) -
BenIshay Zina
Publication year - 1975
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1975.tb02708.x
Subject(s) - bone marrow , cytosine , chemistry , biology , microbiology and biotechnology , immunology , dna , biochemistry
The in vivo effect of a single i.p. injection of cytosine arabinoside (250 mg/kg body weight) on the rat bone marrow is reported. Cytosine arabinoside (ara‐C), a potent inhibitor of DNA‐synthesis, was seen to produce selective necrosis of fast cycling bone marrow erythroblasts. The depleted erythroid marrow is replenished by stem cells which are members of the lymphoid‐transitional cells compartment. An indirect correlation between the degree of erythroid cell depletion and the extent of marrow lymphocytosis was noted 6–12 h after the administration of ara‐C. At 24–48 h, however, the marrow returned to its normal activity. The rat bone marrow 6–12 h following a single injection of ara‐C is suggested as an experimental model for providing stem cells committed to the erythroid line.