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Integrity of the Iron Transport Process in Mice with X‐Linked Anaemia
Author(s) -
Thomson A. B. R.,
Valberg L. S.
Publication year - 1975
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1975.tb02706.x
Subject(s) - absorption (acoustics) , iron deficiency , chemistry , cobalt , metal , duodenum , medicine , ferrous , metabolism , endocrinology , radiochemistry , biophysics , biochemistry , materials science , inorganic chemistry , biology , organic chemistry , composite material , anemia
The defect in iron (Fe) absorption in X‐linked anaemia ( sla ) remains an enigma; absorption of a tracer dose of Fe is impaired in mice raised on an iron‐containing cube diet but not in those raised on an iron‐deficient diet. Because cobalt (Co) shares a similar intestinal transport pathway with Fe, a study was made of the effect of iron deficient diet on Co absorption. The duodenum of sla and genetically normal mice was perfused for 30 min with labelled solutions containing Co or Fe. Co uptake and transfer were similar in sla and normals fed cubes whereas Fe uptake and transfer were less in sla than in normals. The iron deficient diet caused an increase in the uptake and transfer of Co and Fe in sla and normals. When Co and Fe were perfused together in sla fed deficient diet, the uptake and transfer of each metal was less than when perfused alone. The distribution of Fe and Co in subcellular mucosal fractions was determined by a differential centrifugation technique. Deficient diet resulted in a directionally similar change in the subcellular dstribution of Co and Fe in sla and normals. The increase in Co as well as Fe absorption in the sla on an iron deficient diet to the same high level found in genetically normal animals, and the inhibitory effect of each metal on the absorption of the other suggests that the absorption defect in sla is unlikely to be due to a primary defect in the function of the transport carrier.