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Human Fetal Endothelial Cells in Culture
Author(s) -
Henriksen Tore,
Evensen Stein A.,
Elgjo Ragna Følling,
Vefling Anne
Publication year - 1975
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1975.tb02422.x
Subject(s) - umbilical cord , collagenase , umbilical vein , cell culture , biology , andrology , fetus , endothelium , endothelial stem cell , thymidine , fibroblast , cell growth , cell division , immunology , cell , microbiology and biotechnology , endocrinology , medicine , in vitro , biochemistry , pregnancy , genetics , enzyme
Human endothelial cells were isolated from the umbilical cord vein by collagenase treatment and cultured for periods up to 6 weeks. The cultured cells were identified as endothelium by cell morphology and growth pattern, the presence of Weibel‐Palade bodies, and their ability to stimulate allogeneic lymphocytes (Hirschberg et al 1974). Cultured fibroblast‐like cells derived from the umbilical cord were clearly different in all three respects. Approximately one third of the primary endothelial cultures showed clear evidence of proliferation during the first 3–4 days in culture as judged by cell counting. Replicating ability in a culture was correlated with cell density at the time of seeding. Autoradiography of endothelial cells after exposure to 3 H‐thymidine showed a 30‐fold increase in nuclear labelling from day 1 to day 3 in culture. The endothelial cells have so far been subcultured three times.